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生物工程系

高仁钧

日期:2020-08-11 点击数: 来源:

姓名:

高仁钧

职位:

教授

最高学位:

博士

Email:

gaorj@jlu.edu.cn

电话:

13843142825

工作地点:

生命科学楼233

研究方向:

1) 新酶筛选与开发,特别关注嗜热酶的筛选。

2) 基于蛋白质工程的酶的分子改造与固定化。

3) 酶反应器设计、活性调控、工程化制备和酶催化应用。

教育经历:

1992.09-1996.07 吉林大学分子生物学系分子生物学专业, 获学士学位

1998.09-2003.12 吉林大学生命科学学院,获博士学位

2005.09-2007.08 中科院长春应用化学研究所和海王生物 博士后

工作经历:

2000.04-2000.07 日本工业技术院生命工学研究所,访问学者

2001.09-2002.08 吉林大学分子酶学工程教育部重点实验室,助教

2002.09-2004.08 吉林大学分子酶学工程教育部重点实验室,讲师

2004.09-2009.08 吉林大学生命科学学院分子酶学工程教育部重点实验室,副教授

2009.09-现在 吉林大学生命科学学院分子酶学工程教育部重点实验室,教授, 博士生导师

2010.09-2011.09 丹麦奥胡斯大学分子生物学系,访问学者

2018.01-2018.01丹麦奥胡斯大学分子生物学系,访问学者

荣誉称号:

中国生物化学与分子生物学学会工业生物化学分会常务理事

吉林省生物化学与分子生物学会 理事

研究成果:

承担的项目:

国家自然科学基金:嗜热酰基氨肽酶非特异性催化机制的研究及改造

吉林省自然科学基金:高温糖基化生物反应器的构建

国家重点研发计划项目子课题:基于酶和工程菌改造技术的分布式DNA活字存储阵列研究

2017年以来课题组发表文章:

[1] Fang Wang, Yuchen Liu, Chang Du and Renjun Gao*, Current Strategies for Real-Time Enzyme Activation, Biomolecules, 2022, 12, 599.

[2] Jiabao Lyu, Jian Zhang, Jingxuan Zhu, Siao Chen, Tao Han, Yan Zhang, Renjun Gao, Guiqiu Xie*, Zheng Guo*, Molecular dynamics simulation guided distal mutation of Thermotoga naphthophila β-glucosidase for significantly enhanced synthesis of galactooligosaccharides and expanded product scope,International Journal of Biological Macromolecules, 2022, 210, 21-32.

[3] Xiaowen Zhang, Zihao Huang, Dan Wang, Yan Zhang, Bekir Engin Eser, Zhenyu Gu, Rongrong Dai, Renjun Gao*, Zheng Guo*, A new thermophilic extradiol dioxygenase promises biodegradation of catecholic pollutants,Journal of Hazardous Materials, 422 (2022), 126860.

[4] Li Zong, Yan Zhang, Zhengkang Shao, Yingwu Wang, Zheng Guo, Renjun Gao*, Bekir Engin Eser*, Optimization and Engineering of a Self-Sufficient CYP102 Enzyme from Bacillus amyloliquefaciens towards Synthesis of In-Chain Hydroxy Fatty Acids, Catalysts 2021, 11(6), 665.

[5] Li Zong, Renjun Gao, Zheng Guo, hengkang Shao, Yingwu Wang*, Bekir EnginEser*, Characterization and modification of two self-sufficient CYP102 family enzymes from Bacillus amyloliquefaciens DSM 7 with distinct regioselectivity towards fatty acid hydroxylation, Biochemical Engineering Journal, 2021, 166, 107871.

[6] Jiabao Lyu, Renjun Gao, and Zheng Guo*, Galactosyldiacylglycerols: From a Photosynthesis-Associated Apparatus to Structure-Defined In Vitro Assembling, Publication Date: April 1, 2021, https://doi.org/10.1021/acs.jafc.1c00204.

[7] Fang Wang, Rong Li, Hui Jian, Zihao Huang, Yingwu Wang*, Zheng Guo*, and Renjun Gao*, Design and construction of an effective expression system with aldehyde tag for site-specific enzyme immobilization,Catalysts, 2020, 10(4), 410.

[8] Dongni Liu, Lin Deng, Dan Wang, Wei Li, Renjun Gao*,“Bridge regions” regulate catalysis and protein stability of acylpeptide hydrolase, Biochemical Engineering Journal, 2019, 145, 42-52.

[9] Wei Li, Dongni Liu, Xu Geng, Zhengqiang Li* and Renjun Gao*, Real-time regulation of catalysis by remotecontrolled enzyme-conjugated gold nanorod composites for aldol reaction-based applications, Catalysis Science & Technology, 2019, 2221-2230.

[10] Ye Zhou, Bianca Pérez, Weiwei Hao, Jiabao Lv, Renjun Gao*, Zheng Guo*, The additive mutational effects from surface charge engineering: A compromise between enzyme activity, thermostability and ionic liquid tolerance, Biochemical Engineering Journal, 2019, 148,195-204.

[11] Ye Zhou, Nykola C. Jones, Jannik Nedergaard Pedersen, Bianca Pérez, S. V. Hoffmann, Steen Vang Petersen, Jan Skov Pedersen, Adam Perriman, Peter Kristensen, Renjun Gao*, and Zheng Guo*, Insight into the Structure and Activity of Surface-Engineered Lipase Biofluids, ChemBioChem, 2019, 20, 1266-1272.

[12] Jiabao Lyu, Zidan Li, Juncheng Men, Ruyun Jiang, Guilin Tang, Ye Zhou, Renjun Gao*, Covalent immobilization of Bacillus subtilis lipase A on Fe3O4 nanoparticles by aldehyde tag: An ideal immobilization with minimal chemical modification, Process Biochemistry, 2019, 81, 63-69.

[13] Dongni Liu, Wei Li, Xiaoyu Jiang, Shuju Bai, Jiyang Liu, Xutong Liu, Yuhua Shi, Ziyu Kuai, Wei Kong, Renjun Gao* and Yaming Shan*, Using near-infrared enhanced thermozyme and scFv dual-conjugated Au nanorods for detection and targeted photothermal treatment of Alzheimer’s disease, Theranostics, 2019, 9(8), 2268-2281.

[14] Jingwen Yang, Renjun Gao, Ye Zhou, Sampson Anankanbil, Jingbo Li, Guiqiu Xie*, Zheng Guo*, β-Glucosidase from Thermotoga naphthophila RKU-10 for exclusive synthesis of galactotrisaccharides: Kinetics and thermodynamics insight into reaction mechanism, Food Chemistry, 2018, 240, 1:422-429

[15] Jingwen Yang, Bianca Pérez, Sampson Anankanbil, Jingbo Li, Ye Zhou, Renjun Gao*, Zheng Guo*, Valorizing Dairy Waste: Thermophilic Biosynthesis of a Novel Ascorbic Acid Derivative, Journal of Agricultural and Food Chemistry, 2017, 65(41): 9087-9093.

[16] Jingwen Yang, Qi Wang, Ye Zhou, Jingbo Li, Renjun Gao*, Zheng Guo*, Engineering T. naphthophila β-glucosidase for enhanced synthesis of galactooligosaccharides by site-directed mutagenesis, Biochemical Engineering Journal, 2017, 127, 15:1-8.

[17] Jingwen Yang,Bianca Pérez, Sampson Anankanbil,Jingbo Li, Renjun Gao* and Zheng Guo*, Enhanced Synthesis of Alkyl Galactopyranoside by Thermotoga naphthophila β-Galactosidase Catalyzed Transglycosylation: Kinetic Insight of a Functionalized Ionic Liquid-Mediated System, ACS Sustainable Chem. Eng. 2017, 5, 2006-2014.

[18] Ye Zhou, Guiqiu Xie, Lin Chang, Yan Wang, Renjun Gao*, Identification of an archaeal maltooligosyltrehalose trehalohydrolase encoded by an interrupted gene, Extremophile, 2017, 21:537-549.