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师资队伍
高印
基本情况
姓名: 高印
性别:
职称: 副教授
最高学历: 研究生
最高学位: 博士
Email:
详细情况
所在学科专业: 糖生物化学
所研究方向: 目前课题组相关研究包括:(1)聚糖在癌细胞增殖、转移和凋亡中的作用。(2)特异性糖基转移酶抑制素的抗癌活性及机理研究。(3)气体信号分子H2S和NO相互作用机理研究及新型降压药物的研发。(4)透明质酸(玻尿酸)衍生物的合成及抗炎抗癌活性研究。(5)超分子发光材料在生物成像中的应用研究。(6)透明质酸、环糊精和瓜环等药物递送系统的应用研究。
讲授课程: 本科课程:(1)海外精品课-糖生物化学
(2)专业外语 (3)创新实验
研究生课:(1)蛋白质组学
教育经历: 2008.12:学士, 生物化学, 加拿大曼尼托巴大学
2013.07:博士, 生物化学, 加拿大女王大学
工作经历: 2013.07-2015.07: 博士后, 化学, 加拿大女王大学
2015.09-:副教授, 吉林大学生命科学学院
科研项目: 糖生物化学是自然科学中较新的领域也是现今蓬勃发展的领域之一,与基础研究、生物医学、生物技术和药物研发等诸多领域具有广泛的相关性。该领域研究包括聚糖形成和降解的酶学、蛋白与糖之间的相互作用和细胞信号的传导、聚糖在复杂生物系统中的作用,以及通过各种技术对聚糖结构和功能的解析。

多核核磁共振1H-NMR, 13C-NMR, 15N-NMR, 17O-NMR, 19F-NMR, 31P-NMR等和二维核磁共振2D-COSY, 2D-HSQC, 2D-HMBC, 2D-NOESY 技术对于包括材料、生物化学、医药学在内的各个基础科学研究领域都有着重要应用价值,对促进各个学科的交叉与发展都起重要的推动作用。

目前获得资助情况如下:
国家自然科学青年基金 (31700713)
吉林省科技厅青年项目(20170520034JH)
吉林省人才项目(802160100413)

学术论文: 主要研究方向为糖生物学在癌症和炎症研究中的应用和多核核磁共振在化学生物学中的应用。
代表性成果可总结为以下几个方面:
(1) 聚糖结构在癌细胞中的变异及功能的研究。与法国里尔第一大学Catherine Robbe-Masselot教授合作,致力于癌细胞蛋白糖基化的特征并寻找潜在的癌细胞生物标志物的研究。利用MALDI-TOT质谱方法研究癌细胞中糖蛋白糖链结构。阐明了聚糖结构在癌细胞中的变化以及对癌细胞的生长凋亡平衡的影响。这项基础研究对解读癌细胞的产生和转移具有重要的意义,对重新激发癌细胞程序化死亡提供了理论依据。
(2)糖基转移酶的活性和抑制素的研发。与南开大学生命科学院王磊教授合作,从事致命大肠杆菌O157的O-抗体糖基转移酶的研究,通过质谱与核磁共振的手段解析酶产物的结构, 为选择针对性抗菌药的研究奠定了基础。与加拿大女王大学化学系Walter A. Szarek教授长期合作, 研发了一系列糖基转移酶抑制素,其中具有特异性的抑制素能有效抑制糖基转移酶在癌细胞中的生物活性,从而改变癌细胞表面聚糖的结构,例如降低铜绿假单胞菌对肺表面细胞的粘附性。为了更深入的了解抑制素的作用机理,设计并合成了大量与抑制素结构相似的化合物,研究化合物的构效关系。这项研究对新一代消炎药和抗癌药的研发具有重要意义。
(3)多核核磁共振技术研究化学生物学反应物质的本构关系。即用核磁共振技术研究药类化合物装载的宾主系统、药物的化学作用机理以及生物气体信号分子NO和H2S之间的相互作用机理。运用多核核磁共振技术结合紫外、红外光谱以及理论计算解决了一系列困扰化学生物学领域的难题。在同位素标记化合物的合成、核磁共振波谱分析方面积累了丰富的经验。在过去的几年中,已成功解决了化学界对硝酰基化合物化学性质及活性结构的争议;解决了困扰化学界一百七十年之久的格梅林反应Gmelin reaction化学品色现象的争议,该论文被选为期刊代表性文章, 并被美国化学会《C&EN News》报道;构建直接检测降压药硝普钠sodium nitroprusside和2-巯基丁二酸 mercaptosuccinic acid反应的不稳定“红色产物”的方法,对此类降压药物的生物作用机理提供了更深入的认识,论文被期刊主编评选为热点文章。
代表作:
(1)Yin Gao, Yizhe Dai, Gang Wu, Solid-State N and O NMR Studies of S-Nitrosothiols, The Jouranl of Physical Chemistry B, 2017, 121(30):7311-7317.
(2)Yin Gao, Brendan Mossing, Gang Wu, Direct NMR detection of the unstable “red product” from reaction between nitroprusside and 2-mercaptosuccinic acid, Dalton Transactions, 2015, 44(47): 20338-20343.
(3)Yin Gao, Abouzar Toubaei, Xianqi Kong, Gang Wu, Solving the 170-Year-Old mystery about red-violet and blue transient intermediates in the Gmelin reaction, European Journal of Chemistry, 2015, 21: 17172-17177.
- This publication has been highlighted in C&EN News (By Stu Borman, “Nitroprusside Color Mystery Resolved” October 26, 2015).
(4)Yin Gao, Abouzar Toubaei, Xianqi Kong, Gang Wu, Acidity and hydrogen exchange dynamics of iron(II)-bound nitroxyl in aqueous solution, Angewandte Chemie International Edition, 2014, 53(43): 11547-11551.
(5)Yin Gao, Rajindra P. Aryal, Tongzhong Ju, Richard D. Cummings, Gagandeep Gahlay, Donald L. Jarvis, Khushi Matta, Jason Z. Vlahakis, Walter A. Szarek, Inka Brockhausen*, Acceptor specificities and selective inhibition of recombinant human Gal- and GlcNAc-transferases that synthesize core structures 1, 2, 3 and 4 of O-glycans, Biochimica et Biophysica Acta, 2013, 1830(8): 4274-4281.
(6)Yin Gao, Jason Z. Vlahakis, Walter Szarek, Inak Brockhausen, Selective inhibition of glycosyltransferases by bivalent imidazolium salts. Bioorganic & Medicinal Chemistry, 2013, 21(5): 1305-1311.
(7)Yin Gao, Bin Liu, Scott Strum, John S. Schutzbach, Tatyana N. Druzhinina, Natalia S. Utkina, Vladimir I. Torgov, Leonid L. Danilov, Vladimir V. Veselovsky, Jason Z. Vlahakis, Walter A. Szarek, Lei Wang, Inka Brockhausen, Biochemical characterization of WbdN, a β1,3-glucosyltransferase involved in O-antigen synthesis in enterohemorrhagic Escherichia coli O157, Glycobiology, 2012, 22(8): 1092 -1102.
(8)Yin Gao, Vishwanath Chachadi, Pi-Wan Cheng, Inka Brockhausen, Glycosylation potential of human prostate cancer cell lines. Glycoconjugate Journal, 2012, 29(7): 525-537.
(9)Yin Gao, Carmen Lazar, Walter A. Szarek, Inka Brockhausen, Specificity of β1,4-galactosyltransferase inhibition by 2-naphthyl 2-butanamido-2-deoxy-1-thio-β-D-glucopyranoside, Glycoconjugate Journal, 2010, 27(7): 673 -684.
会议:
(1) Yin Gao, Scott Strum, Bin Liu, John S. Schutzbach, Tatyana N. Druzhinina, Natalia S. Utkina, Vladimir I. Torgov, Walter A. Szarek, Lei Wang and Inka Brockhausen, "O-antigen synthesis in enterohaemorrhagic Escherichia coli serotype O157: characterization of a novel glucosyltransferase", Society for Glycobiology Annual Meeting, Seattle, WA, United States, Nov 9 – 12, 2011. (Oral).
(2)Yin Gao, Catherine Robbe-Masselot, Gregory Lee and Inka Brockhausen, “Structures of Carbohydrate-associated epitope on CA215 expressed in Ovarian and Cervical cancer cell lines and proposed Biosynthetic Pathways”, 244th ACS National Meeting & Exposition, Philadelphia, PA, United States, Aug 17 – 23, 2012. (Oral).
(3) Yin Gao and Harry W. Duckworth, “The role of 3 AA side chains in the function of Escherichia coli citrate synthase”, The 91st Canadian Chemistry Conference and Exhibition, Edmonton, Alberta, Canada, May 24 – 28, 2008. (Poster).
(4) Yin Gao and Inka Brockhausen, “Glycosylation pathways of human prostate cancer cells”, 20th International Symposium on Glycoconjugates, San Juan, Puerto Rico, United States, Nov 29 – Dec 4, 2009. (Poster).
(5) Yin Gao, Changchang Xu, Karen J. Rees-Milton, Inka Brockhausen and Tassos A. Anastassiades, “Glycosyltransferases and acyltransferases in bone and joint disease”, Canadian Connective Tissue Conference, Toronto, Ontario, Canada, June 3 – 5, 2010. (Poster).
(6) Yin Gao, Pi-Wan Cheng, Yifan Wang, Tassos A. Anastassiades and Inka Brockhausen, “The characteristic glycosylation potential of human prostate cancer cells”, Society for Glycobiology Annual Meeting, St. Petersburg, Florida, United States, Nov 7 – 10, 2010. (Poster).
(7) Yin Gao, Scott Strum, Bin Liu, John S. Schutzbach, Tatyana N. Druzhinina, Natalia S. Utkina, Vladimir I. Torgov, Walter A. Szarek, Lei Wang and Inka Brockhausen, "Characterization of a novel glucosyltransferase WbdN from Escherichia coli seroptype O157", 21st International Symposium on Glycoconjugates, Vienna, Austria, Aug 21 – 26, 2011. (Poster).
(8) Yin Gao, Jason Z. Vlahakis, Walter A. Szarek and Inka Brockhausen, "Imidazolium compounds: A new generation of galactosyltransferase inhibitors", Society for Glycobiology Annual Meeting, Seattle, WA, United States, Nov 9 – 12, 2011. (Poster).
(9) Inka Brockhausen, Yin Gao, Bin Liu, Jason Z. Vlahakis, Walter A. Szarek and Lei Wang, “O-antigen synthesis in enterohaemorrhagic Escherichia coli: Characterization of novel glycosyltransferases”, International Symposium on Glycosyltransferases, Hannover, Germany, June 5 – 9, 2012. (Poster).
(10) Yin Gao, Catherine Robbe-Masselot, Gregory Lee and Inka Brockhausen, “Structures of Carbohydrate-associated Epitope on CA215 Expressed in Ovarian and Cervical Cancer Cell lines”, International Symposium on Glycosyltransferases, Hannover, Germany, June 5 – 9, 2012. (Poster).
(11) Inka Brockhausen, Yin Gao, Bin Liu, Jason Z. Vlahakis, Walter A. Szarek and Lei Wang, “O-antigen synthesis in enterohaemorrhagic Escherichia coli: Characterization of novel glycosyltransferases”, 243th ACS National Meeting & Exposition, San Diego, CA, United States, March 25 – 29, 2012. (Poster).
(12) Yin Gao, Jiasheng Lu, Xianqi Kong and Gang Wu, “Synthesis of myoglobin-HNO adducts”, MOOT XXVI NMR Symposium, Kingston, ON, Canada, Oct 25 – 27, 2013. (Poster).
着作教材: (1)Yin Gao, Anna Vinnikova, Inka Brockhausen, Functional identification of bacterial glucosyltransferase WbdN, Methods. Mol. Biol., 2013, 1022: 199 – 214, Springer Science+Business Media New York, 2013
(2)Inka Brockhausen, Yin Gao, Chapter 8. Structural glycobiology: applications in cancer research, CRC Press, Taylor & Francis Group (Print ISBN: 978-1-4398-5460-0), 2012
治学格言: 淡泊明志,宁静致远。

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